Recombinant TNFSF15 antibody

Catalog No. ABIN7661139

This anti-TNFSF15 antibody is a Mouse Monoclonal antibody detecting TNFSF15 in IHC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant TNFSF15 antibody (ABIN7661139)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF15 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RVEGI-1283

Product Details anti-TNFSF15 Antibody

Purpose: TNFS15 / VEGI (Vascular Endothelial Growth Inhibitor) (rVEGI/1283)

Characteristics: VEGI is an anti-angiogenic cytokine that belongs to tumor necrosis factor superfamily, member 15 (TNFSF15). This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Reduced expression of VEGI has been reported as a marker of poor prognosis in breast cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human VEGI protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Endothelial cells. Colon, intestine, placenta, lung, liver, kidney, pancreas, spleen and prostate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TNFSF15

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: TNFS15

Background:

Synonyms: TNF ligand-related molecule 1 (TL1A), Tumor necrosis factor (ligand) superfamily member 15 (TNFSF15), Vascular endothelial growth inhibitor 192a, Vascular endothelial growth inhibitor (VEGI), VEGI192A

Gene Symbol: TNFSF15

Tissue Expression: Endothelial cells

Molecular Weight: 22 kDa

Gene ID: 9966

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy