Recombinant TYR antibody

Catalog No. ABIN7661479

This Rabbit Monoclonal antibody specifically detects TYR in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant TYR antibody (ABIN7661479)

Target: TYR (Tyrosinase (TYR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: TYR-2024R

Product Details anti-TYR Antibody

Purpose: Tyrosinase (Melanoma Marker) (TYR/2024R)

Characteristics: This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human tyrosinase (TYR) protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 or 10 mM citrate buffer pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: SK-MEL-19, SK-MEL-30 cells or Melanoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: Tyrosinase

Background:

Synonyms: ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR

Gene Symbol: TYR

Tissue Expression: Melanocytes

Molecular Weight: 70-80 kDa

Gene ID: 7299

UniProt: P14679