Ubiquitin antibody (AA 1-119) (CF®740)

Catalog No. ABIN7661581

This Mouse Monoclonal antibody specifically detects Ubiquitin in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Ubiquitin antibody (AA 1-119) (CF®740) (ABIN7661581)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: UBB-2122

Product Details anti-Ubiquitin Antibody (CF®740)

Binding Specificity: AA 1-119

Purpose: Ubiquitin (Autophagy Marker) (UBB/2122), CF740 conjugate

Characteristics: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119)

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa or Jurkat cells. Alzheimer s Brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background:

Synonyms: Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B

Gene Symbol: UBB

Molecular Weight: 9 kDa

Gene ID: 7314

UniProt: P0CG47, P0CG48, P62979, P62987, P62988

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway