MYB antibody (AA 119-135) (CF®740)

Catalog No. ABIN7661767

This Mouse Monoclonal antibody specifically detects MYB in . It exhibits reactivity toward Human.

Quick Overview for MYB antibody (AA 119-135) (CF®740) (ABIN7661767)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYB antibody is conjugated to CF®740

Application: Please inquire

Clone: MYB286

Product Details anti-MYB Antibody (CF®740)

Binding Specificity: AA 119-135

Purpose: c-Myb(MYB286), CF740 conjugate

Characteristics: The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-terminal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-myb oncoprotein, coupled to KLH

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Jurkat, MOLT-4, COLO or BT-20 cells. Colon or breast carcinomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: c-Myb

Background:

Synonyms: c-myb_CDS, efg, ONCOGENE AMV, Oncogene MYB, Proto-oncogene c-Myb, Transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog

Gene Symbol: MYB

Molecular Weight: 75 kDa

Gene ID: 4602

UniProt: P01104

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process