VEGF antibody (CF®568)

Catalog No. ABIN7661840

The Mouse Monoclonal anti-VEGF antibody has been validated for WB, IHC, IHC (fp) and Func. It is suitable to detect VEGF in samples from Human.

Quick Overview for VEGF antibody (CF®568) (ABIN7661840)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGF antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Functional Studies (Func)

Clone: VG76e

Product Details anti-VEGF Antibody (CF®568)

Purpose: VEGF (Vascular Endothelial Growth Factor)(VG76e), CF568 conjugate

Characteristics: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human VEGF189 recombinant protein

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order Ab without BSA, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Background:

Synonyms: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA

Gene Symbol: VEGFA

Tissue Expression: Endothelial cells

Molecular Weight: 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)

Gene ID: 7422

UniProt: P15692