WIPI2 antibody (AA 418-436) (CF®740)

Catalog No. ABIN7662032

The Mouse Monoclonal anti-WIPI2 antibody has been validated for WB, IP and IF. It is suitable to detect WIPI2 in samples from Human and Mouse.

Quick Overview for WIPI2 antibody (AA 418-436) (CF®740) (ABIN7662032)

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This WIPI2 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 2A2

Product Details anti-WIPI2 Antibody (CF®740)

Binding Specificity: AA 418-436

Purpose: WIPI2(2A2), CF740 conjugate

Characteristics: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al. , 2004 ). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 418-436) of human WIPI2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: HEK293 cells. Human or mouse placenta, skeletal muscle or testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for WIPI2

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Alternative Name: WIPI2

Background:

Synonyms: ATG18B, Atg21, CGI 50, DKFZp434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WD repeat domain phosphoinositide-interacting protein 2, WIPI2, WIPI49 like protein 2

Gene Symbol: WIPI2

Molecular Weight: 49 kDa

Gene ID: 26100

UniProt: Q9Y4P8