SIRPA antibody

Catalog No. ABIN7670119

The Rat Monoclonal anti-SIRPA antibody has been validated for FACS. It is suitable to detect SIRPA in samples from Mouse.

Quick Overview for SIRPA antibody (ABIN7670119)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: P84

Product Details anti-SIRPA Antibody

Purpose: Purified Anti-Mouse CD172a Antibody[P84]

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: ≥ 1 mg/mL

Buffer: Phosphate buffered solution, pH 7.2, containing 0.05 % proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze / thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a

Background: Myd;SHPS;Ptpns;Bit;Myd1;Ptpns1;Shps1;Sirp;Sirpa; SIRP&beta-1-1;SIRPαSIRPβAI835480;CD172a;P84;SHP-1;SHPS-1,Immunoglobulin-like cell surface receptor for CD47. Acts as a docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization. Receptor for THBS1. Interaction with THBS1 stimulates phosphorylation of SIRPA. In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production.

UniProt: P97797