NUMA1 antibody

Catalog No. ABIN7670700

The Mouse Monoclonal anti-NUMA1 antibody has been validated for ELISA (Capture). It is suitable to detect NUMA1 in samples from Human.

Quick Overview for NUMA1 antibody (ABIN7670700)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: ELISA (Capture)

Clone: 2F1

Product Details anti-NUMA1 Antibody

Purpose: NUMA/NMP22 Monoclonal Antibody(Capture)

Purification: Protein A/G Purification

Immunogen: Recombinant Human NUMA/NMP22 protein expressed by E.coli

Isotype: IgG2a

Application Details

Application Notes: ELISA Capture 2-8 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Preservative: Without preservative

Handling Advice: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Expiry Date: 12 months

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NUMA/NMP22

Background: NucLear mitotic apparatus protein 1,NucLear matrix protein-22(NMP-22),NucLear mitotic apparatus protein,SP-H antigen,NUMA1,NUMA,Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division.Functions to tether the minus ends of MTs at the spindle poles,which is critical for the establishment and maintenance of the spindle poles.Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner.In metaphase,part of a ternary complex composed of GPSM2 and G(i) alpha proteins,that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles,and hence regulates the correct oritentation of the mitotic spindle.During anaphase,mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2),and hence participates in the regulation of the spindle elongation and chromosome segregation.Binds also to other polyanionic phosphoinositides,such as phosphatidylinositol 3-phosphate (PIP),lysophosphatidic acid (lPA) and phosphatidylinositol triphosphate (PIP3),in vitro.Also required for proper orientation of the mitotic spindle during asymmetric cell divisions.Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role,occupies the majority of the nuclear volume. Required for epidermal differentiation and hair follicle morphogenesis.

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase