S100A9 antibody

Catalog No. ABIN7670737

This anti-S100A9 antibody is a Rabbit Polyclonal antibody detecting S100A9 in ELISA. Suitable for Human.

Quick Overview for S100A9 antibody (ABIN7670737)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: ELISA

Product Details anti-S100A9 Antibody

Purpose: S100A9 Polyclonal Antibody(Capture/Detector)

Purification: Antigen Affinity Purification

Immunogen: Recombinant Human S100A9 protein expressed by E.coli

Isotype: IgG

Application Details

Application Notes: ELISA Capture 2-8 μg/mL,ELISA Detector 0.1-0.4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Preservative: Without preservative

Handling Advice: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Expiry Date: 12 months

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9

Background: Protein S100-A9,CaLgranuLin-B, CaLprotectin L1H subunit, Leukocyte L1 compLex heavy chain,Migration inhibitory factor-reLated protein 14,MRP-14,p14,S100 caLcium-binding protein A9,S100A9,CAGB,CFAG,MRP14,S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis,adhesion,can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK,PI3K/AKT,and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism,modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase . Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane,transferring arachidonic acid,an essential cofactor,to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory,antimicrobial,oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes,promotion of cytokine and chemokine production,and regulation of leukocyte adhesion and migration . Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TlR4) and receptor for advanced glycation endproducts (AGER). Binding to TlR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins