E2F5 antibody (AA 101-200)

Catalog No. ABIN733673

The Rabbit Polyclonal anti-E2F5 antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect E2F5 in samples from Human and Rat. There is 1 publication available.

Quick Overview for E2F5 antibody (AA 101-200) (ABIN733673)

Target: E2F5

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-E2F5 Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human E2F5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-E2F5 antibody (ABIN733673)

Zhao, Wu, Ling, Lin, Fu, Deng, He, Zhong: "Analysis of genetic aberrations on chromosomal region 8q21-24 identifies E2F5 as an oncogene with copy number gain in prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 465, (2013) (PubMed).

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5

Background:

Synonyms: E2F-5, Transcription factor E2F5, E2F5

Background: Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Gene ID: 1875

UniProt: Q15329