WNT1 antibody (AA 251-350)

Catalog No. ABIN733748

There are 4+ publications for this product available. The Rabbit Polyclonal anti-WNT1 antibody is suitable to detect WNT1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for WNT1 antibody (AA 251-350) (ABIN733748)

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-WNT1 Antibody

Binding Specificity: AA 251-350

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Wnt1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-WNT1 antibody (ABIN733748)

Chen, Chen, Wallace, Chen, Yang, Sung, Chiang, Chen, Chua, Yip, Cheng: "Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome." in: American journal of translational research, Vol. 9, Issue 2, pp. 214-229, (2017) (PubMed).

Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Gao, Yang, Gao, Xu, Niu, Li, Wen: "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." in: Molecular medicine reports, Vol. 12, Issue 2, pp. 1971-6, (2015) (PubMed).

Gao, Liu, Chen, Lv, Wu, Mi, Wang: "Comparative study of Hsp27, GSK3?, Wnt1 and PRDX3 in Hirschsprung's disease." in: International journal of experimental pathology, Vol. 95, Issue 3, pp. 229-37, (2014) (PubMed).

Target Details for WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: Wnt1

Background:

Synonyms: INT1, OI15, BMND16, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, WNT1

Background: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development.

Gene ID: 7471

UniProt: P04628

Pathways: WNT Signaling, Dopaminergic Neurogenesis