DDIT4 antibody

Catalog No. ABIN7673012

This Rabbit Polyclonal antibody specifically detects DDIT4 in WB, ELISA, IHC and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DDIT4 antibody (ABIN7673012)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-DDIT4 Antibody

Purpose: DDIT4-specific antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: DNA-damage-inducible transcript 4

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:200-1:2000, IHC: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: DDIT4

Background:

Synonyms: DNA damage-inducible transcript 4 protein|HIF-1 responsive protein RTP801|Protein regulated in development and DNA damage response 1 (REDD-1)|DDIT4|REDD1|RTP801

Background: REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family.Its function:Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1(mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type, it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes(By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication(By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.This antibody is specific to the REDD1.

Molecular Weight: 35 kDa

Gene ID: 54541

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process