GFP Tag antibody

Catalog No. ABIN7673315

This Rabbit Polyclonal antibody specifically detects GFP Tag in WB, ELISA and IP. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP Tag antibody (ABIN7673315)

Target: GFP Tag

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-GFP Tag Antibody

Purpose: GFP tag antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: full length

Immunogen: GFP tag

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:4000, IP: 1:2000-1:20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for GFP Tag

Target: GFP Tag

Target Type: Tag

Background:

Synonyms: CFP|eGFP|eYFP|GFP|GFP tag|YFP

Background: Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9 kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

Molecular Weight: 30 kDa