MBP Tag antibody

Catalog No. ABIN7673460

The Mouse Monoclonal anti- antibody has been validated for WB, ELISA and IP. It is suitable to detect in samples from .

Quick Overview for MBP Tag antibody (ABIN7673460)

Target: MBP Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 0D8

Product Details

Purpose: MBP tag antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: MBP-Tag

Isotype: IgG2a

Application Details

Application Notes: WB: 1:1000-1:8000, IP: 1:5000-1:50000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details

Target: MBP Tag

Target Type: Tag

Background:

Synonyms: Maltose Binding Protein|MBP|MBP Tag

Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.

Molecular Weight: 40 kDa