MCAM antibody (PE)

Catalog No. ABIN7673692

This anti-MCAM antibody is a Rat Monoclonal antibody detecting MCAM in FACS. Suitable for Mouse.

Quick Overview for MCAM antibody (PE) (ABIN7673692)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: ME-9F1

Product Details anti-MCAM Antibody (PE)

Purpose: PE Anti-Mouse CD146 Antibody

Purification: 0

Purity: 0

Isotype: IgG2a, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 % BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background:

Synonyms: S-Endo 1 antigen|MUC18|MCAM|Mel-CAM|A32 antigen

Background: CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30 % of neutrophils and 60 % of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

Molecular Weight: 0

Gene ID: 84004

UniProt: Q8R2Y2