TUBB3 antibody

Catalog No. ABIN7674576

The Mouse Monoclonal anti-TUBB3 antibody is suitable to detect TUBB3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for TUBB3 antibody (ABIN7674576)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 2A10

Product Details anti-TUBB3 Antibody

Purpose: TUBB3-specific antibody

Purification: protein A+G purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: tubulin, beta 3

Isotype: IgG1

Application Details

Application Notes: WB: 1:5000-1:30000, IHC: 1:50-1:300, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3

Background:

Synonyms: Tubulin beta-3 chain|Tubulin beta-4 chain|Tubulin beta-III|TUBB3|TUBB4

Background: TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

Molecular Weight: 50-55 kDa

Gene ID: 10381

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase