UBE2I antibody

Catalog No. ABIN7674581

This Rabbit Polyclonal antibody specifically detects UBE2I in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for UBE2I antibody (ABIN7674581)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-UBE2I Antibody

Purpose: UBE2I-Specific antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: Peptide

Immunogen: ubiquitin-conjugating enzyme E2I(UBC9 homolog, yeast)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IHC: 1:20-1:200,IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I

Background:

Synonyms: SUMO-conjugating enzyme UBC9|RING-type E3 SUMO transferase UBC9|SUMO-protein ligase|Ubiquitin carrier protein 9|Ubiquitin carrier protein I|Ubiquitin-conjugating enzyme E2 I|Ubiquitin-protein ligase I|p18|UBE2I|UBC9|UBCE9

Background: UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 Molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Molecular Weight: 18 kDa

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway