DUSP1 antibody (AA 151-250)

Catalog No. ABIN735368

The Rabbit Polyclonal anti-DUSP1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect DUSP1 in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for DUSP1 antibody (AA 151-250) (ABIN735368)

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUSP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DUSP1 Antibody

Binding Specificity: AA 151-250

Specificity: This antibody may interact with DUSP4/MKP2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DUSP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DUSP1 antibody (ABIN735368)

Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

Kato, Naiki-Ito, Naiki, Suzuki, Yamashita, Sato, Sagawa, Kato, Kuno, Takahashi: "Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis." in: Oncotarget, Vol. 7, Issue 2, pp. 2009-21, (2016) (PubMed).

Khadir, Tiss, Abubaker, Abufarha, Al-Khairi, Cherian, John, Kavalakatt, Warsame, Al-Madhoun, Al-Ghimlas, Elkum, Behbehani, Dermime, Dehbi: "MAP kinase phosphatase DUSP1 is overexpressed in human obese and modulated by physical activity." in: American journal of physiology. Endocrinology and metabolism, pp. ajpendo.00577.2013, (2014) (PubMed).

Target Details for DUSP1

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Alternative Name: DUSP1/MKP1

Background:

Synonyms: HVH1, MKP1, CL100, MKP-1, PTPN10, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, Protein-tyrosine phosphatase CL100, DUSP1, VH1

Background: Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

Gene ID: 1843

UniProt: P28562