DNAJC3 antibody (AA 351-450)

Catalog No. ABIN735548

There is 1 publication for this product available. The Rabbit Polyclonal anti-DNAJC3 antibody is suitable to detect DNAJC3 in samples from Mouse and Rat. It has been validated for ELISA, IF (cc), IF (p), IHC (p) and IHC (fro).

Quick Overview for DNAJC3 antibody (AA 351-450) (ABIN735548)

Target: DNAJC3 (DnaJ (Hsp40) Homolog, Subfamily C, Member 3 (DNAJC3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DNAJC3 Antibody

Binding Specificity: AA 351-450

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human p58ipk/DNAJC3

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DNAJC3 antibody (ABIN735548)

Taguchi, Allende, Mizukami, Cook, Gavrilova, Tuymetova, Clarke, Chen, Olivera, Proia: "Sphingosine-1-phosphate Phosphatase 2 Regulates Pancreatic Islet β-Cell Endoplasmic Reticulum Stress and Proliferation." in: The Journal of biological chemistry, Vol. 291, Issue 23, pp. 12029-38, (2016) (PubMed).

Target Details for DNAJC3

Target: DNAJC3 (DnaJ (Hsp40) Homolog, Subfamily C, Member 3 (DNAJC3))

Alternative Name: DNAJC3

Background:

Synonyms: DNAJC3, Protein Kinase Inhibitor P58, DnaJ homolog subfamily C member 3, HP58, Interferon induced, double stranded RNA activated protein kinase inhibitor, P58, p58 ipk , P58IPK, PRKRI, Protein kinase inhibitor of 58 kDa, Protein kinase inhibitor p58.

Background: DNAJC3 may act as an inhibitor of both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2/PKR to catalyze phosphorylation of the EIF2A. It also functions as a Co-chaperone of HSPA8/HSC70 and is involved in the unfolded protein response (UPR) during ER stress. It has been reported that this protein stimulates the ATPase activity of HSPA8/HSC70.DNAJC3 is also involved in ativiral defense - it is activated in response to infection by influenza virus through the dissociation of DNAJB1. DNAJC3 is widely expressed and can be found at high levels in the testis and pancreas (expression in cell lines is variable).

Gene ID: 5611

UniProt: Q13217

Pathways: ER-Nucleus Signaling