PFN2 antibody (AA 51-140) (HRP)

Catalog No. ABIN735737

The Rabbit Polyclonal anti-PFN2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect PFN2 in samples from Human.

Quick Overview for PFN2 antibody (AA 51-140) (HRP) (ABIN735737)

Target: PFN2 (Profilin 2 (PFN2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PFN2 Antibody (HRP)

Binding Specificity: AA 51-140

Specificity: This antibody may detect Profilin 1 due to a 71 % amino acid sequence similarity in the immunogen sequence.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Profilin 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PFN2

Target: PFN2 (Profilin 2 (PFN2))

Alternative Name: Profilin 2

Background:

Synonyms: Profilin-2, Profilin II, Pfn2

Background: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity).

Gene ID: 81531

UniProt: Q9EPC6

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis