Glutamate Dehydrogenase (GDH) antibody

Catalog No. ABIN7794693

The Mouse Monoclonal anti- antibody has been validated for ELISA. It is suitable to detect in samples from Clostridium difficile.

Quick Overview for Glutamate Dehydrogenase (GDH) antibody (ABIN7794693)

Target: Glutamate Dehydrogenase (GDH)

Reactivity: Clostridium difficile

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA

Clone: CDIF-6581

Product Details

Purpose: Anti-Clostridium difficile, GDH (CDIF-6581)

Purification: This monoclonal antibody is purified by protein A chromatography or sequential differential precipitations.

Purity: >=90 %

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1:20-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 μg/mL

Buffer: Formulated in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1 % sodium azide. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: This purified antibody is stable when stored at 2-8°C. Do not freeze.

Target Details

Target: Glutamate Dehydrogenase (GDH)

Alternative Name: GDH

Background: GDH, glutamate dehydrogenase,?C. diff, C. difficile,Background: Clostridium difficile, often referred to as C. diff, is an anaerobic, Gram-positive bacterium that can cause a range of gastrointestinal issues in humans. Infections with C. difficile are primarily associated with healthcare settings such as hospitals and long-term care facilities where patients come into contact with infected individuals or contaminated surfaces1. C. diff infections (CDIs) typically occur following the disruption of the normal gut microbiota, often due to antibiotics, which allow the bacteria to proliferate and produce toxins2. Glutamate dehydrogenase (GDH) is an enzyme produced by C. difficile which is responsible for conversion of glutamate to alpha-ketoglutarate. GDH detection in the stool is often used as a rapid screening tool to suggest possible presence of C. diff. Further testing for the bacterial toxins is typically performed upon a positive GDH test.