West Nile Virus antibody

Catalog No. ABIN7794789

The Mouse Monoclonal anti-West Nile Virus antibody has been validated for ELISA and FACS. It is suitable to detect West Nile Virus in samples from West Nile Virus (WNV).

Quick Overview for West Nile Virus antibody (ABIN7794789)

Target: West Nile Virus (WNV)

Reactivity: West Nile Virus (WNV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This West Nile Virus antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: WNV-99

Product Details anti-West Nile Virus Antibody

Purpose: Anti-West Nile Virus (Clone: WNV-99)-Purified No Carrier Protein

Specificity: WNV-99 activity is directed against the wing, flexible loop of NS1. During the screening phase and post characterization clone WNV-99 was found to bind to solid-phase recombinant WNV NS1 protein and to NS1 expressed on the surface of intact WNV-infected Vero cells. Plus, also engaged the hexameric form of NS1. WNV-99 was cross-reactive against Japanese Encephalitis Virus and Tick-borne Encephalitis Virus in a direct ELISA using recombinant NS1 proteins. Background West Nile Virus (WNV) is a mosquito-borne, enveloped, positive-stranded RNA flavivirus1. Flavivirus nonstructural protein NS1 has been proposed as an antibody target to avoid antibody-dependent enhancement2. NS1 is a 46-55 kDa glycoprotein that is expressed as a dimer on the cell surface and as a soluble hexamer in the extracellular space and in circulation during infection. WNV NS1 dimer consists of a ?-roll, wing, and ?-ladder.

Purification: Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Purity: >=90 % monomer by analytical SEC and SDS-Page

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1 μg/mL

Buffer: This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Preservative: Without preservative

Storage: 4 °C,-80 °C

Storage Comment: This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at <= -70°C.Avoid Repeated Freeze Thaw Cycles.

Target Details for West Nile Virus

Target: West Nile Virus (WNV)

Alternative Name: West Nile virus

Target Type: Virus