Recombinant CD3 epsilon antibody

Catalog No. ABIN7795311

The Mouse Monoclonal anti-CD3 epsilon antibody has been validated for ELISA, Func, Neut and IGA. It is suitable to detect CD3 epsilon in samples from Human.

Quick Overview for Recombinant CD3 epsilon antibody (ABIN7795311)

Target: CD3 epsilon (CD3E)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: ELISA, Functional Studies (Func), Neutralization (Neut), Immunogenicity Assay (IGA)

Grade: In vivo Grade

Clone: OKT3

Product Details anti-CD3 epsilon Antibody

Expression System: Mammalian cells

Purpose: In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Mouse IgG2a Kappa (Clone: OKT3)

Characteristics: The OKT3 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, OKT3, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the OKT3 antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the OKT3 antibody, T cells disappear from the circulation within minutes to hours. During treatment with the OKT3 antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the OKT3 antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the OKT3 antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the OKT3 antibody in vivo. The OKT3 antibody is ineffective clinically if the CD3 target molecule is not modulated. The OKT3 antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway