Recombinant CD8 alpha antibody

Catalog No. ABIN7795355

This Rat Monoclonal antibody specifically detects CD8 alpha in FACS, IHC, WB, ELISA, ICC and in vivo. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant CD8 alpha antibody (ABIN7795355)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), In vivo Studies (in vivo)

Grade: In vivo Grade

Clone: YTS 105-18

Product Details anti-CD8 alpha Antibody

Expression System: Mammalian cells

Purpose: In vivo Grade Recombinant Anti-mouse CD8a Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 105.18)

Characteristics: The rat anti-mouse CD8a monoclonal antibody YTS 105.18 (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. YTS 105.18 is a rat IgG2b anti-CD8a monoclonal antibody that does not deplete CD8+ T-cells in vivo and is widely used for the blockade of CD8+ T-cell activity in mice. Inoculation of mice with YTS 105.18 induces tolerance to zenograft transplantation and reduces insulin-dependent diabetes mellitus (IDDM) in NOD mice. Some suppliers and researchers state that YTS 105.18 is a rat IgG2a monoclonal antibody. Its sequence of the heavy chain indicates that it is a rat IgG2b antibody. Our recombinant YTS 105.18 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: YTS 105.18).

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Pathways: TCR Signaling