Recombinant CD32/CD16 antibody

Catalog No. ABIN7795362

The Human Monoclonal anti-CD32/CD16 antibody is suitable to detect CD32/CD16 in samples from Mouse. It has been validated for FACS, IHC, WB, ELISA, ICC and in vivo.

Quick Overview for Recombinant CD32/CD16 antibody (ABIN7795362)

Target: CD32/CD16

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Human

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), In vivo Studies (in vivo)

Grade: In vivo Grade

Clone: 2-4G2

Product Details anti-CD32/CD16 Antibody

Expression System: Mammalian cells

Purpose: In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Human IgG1 Kappa (Clone: 2.4G2)

Characteristics: The recombinant 2.4G2 antibody binds to mouse CD16 (FcγIII) and CD32 (FcγII) receptor proteins. The major component of some commercial Fc block reagents is the 2.4G2 antibody. The rat, mouse, and human IgG Fc binds to mouse CD64 (FcγI). Thus, the recombinant 2.4G2 antibodies can be used to block non-specific binding of IgGs to mouse Fc receptors in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. Customers may choose different IgG isotypes for their own applications. The most popular mouse Fc blocking reagents include: Anti-Mouse CD16/CD32 (Clone 2.4G2): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining, it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity. Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: for general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. Purified rat IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls. Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking. Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. Best choice of mouse Fc blocking reagents based on your application are: For flow cytometry: anti-mouse CD16/CD32 (Clone 2.4G2). For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. For Western Blot and ELISA: BSA or FBS. For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/CD32