Recombinant CD370 & CD47 antibody

Catalog No. ABIN7795543

The Mouse Bispecific anti-CD370 & CD47 antibody (Clone 10B4-A4) (ABIN7795543) specifically detects CD370 & CD47 in ELISA, FACS, ICC, IHC, in vivo and WB. The antibody is reactive with Mouse samples.

Quick Overview for Recombinant CD370 & CD47 antibody (ABIN7795543)

Target: CD370 & CD47

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Bispecific

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), In vivo Studies (in vivo), Western Blotting (WB)

Grade: In vivo Grade

Clone: 10B4-A4

Product Details

Expression System: Mammalian cells

Purpose: In Vivo Grade Recombinant Anti-mouse CD370 / CD47 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone 10B4 / A4)

Characteristics: The rat anti-mouse CD370 (Clec9A or DNGR-1) monoclonal antibody 10B4 (rat IgG2a kappa) reacts with the mouse CD370 protein. CLEC9A (C-type lectin domain family 9 member A), a transmembrane glycoprotein with a single extracellular C-type lectin domain (CTLD), plays a role in the immune response. As an activation receptor, CLEC9A is expressed on myeloid lineage cells, helps process material from dead cells, recognizes filamentous actin when cell membranes are damaged, and helps present antigens to cytotoxic T cells. CLEC9A has an intracellular cytoplasmic tail with potential signaling motifs. CLEC9A is expressed on a subset of BDCA3+ dendritic cells and CD16- monocytes in humans, and is expressed on CD8+ conventional DCs and plasmacytoid DCs in mice. It is under investigation how the recombinant anti-mouse CD370 / CD47 bispecific antibodies (10B4 / A4) behave different from the individual monoclonal antibodies and their combination. Our recombinant anti-mouse CD370 / CD47 bispecific antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD370 antibody (hybridoma clone name or number: 10B4) and the rat anti-mouse CD47 antibody (hybridoma clone name or number: A4).

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG2c, L234A, L235A, P329G, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details

Target: CD370 & CD47

Sub Type: Bispecific