Oct-2 antibody (AA 11-100)

Catalog No. ABIN737316

Product Details anti-Oct-2 Antibody

Target: Oct-2 (POU2F2) (POU domain, class 2, transcription factor 2 (POU2F2))

Binding Specificity: AA 11-100

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oct-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse OCT2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Oct-2 antibody (ABIN737316)

Zhou, Bi, Gao, An, Chen, Chen: "Macrophage polarization in human gingival tissue in response to periodontal disease." in: Oral diseases, Vol. 25, Issue 1, pp. 265-273, (2019) (PubMed).

Xu, Zhu, Chan, Lu, Shen, Gillies, Zhou: "The Altered Renal and Hepatic Expression of Solute Carrier Transporters (SLCs) in Type 1 Diabetic Mice." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120760, (2015) (PubMed).

Target Details for Oct-2

Target: Oct-2 (POU2F2) (POU domain, class 2, transcription factor 2 (POU2F2))

Alternative Name: 2-Oct (POU2F2 Products)

Background:

Synonyms: Otf2, Oct-2, Oct2a, Oct2b, Otf-2, POU domain, class 2, transcription factor 2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Octamer-binding transcription factor 2, Pou2f2, Oct2

Background: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform OCT2.5 activates the U2 small nuclear RNA (snRNA) promoter. Isoforms OCT2.1, OCT2.2 and OCT2.3 activate octamer-containing promoters. Isoforms OCT2.4 and OCT2.5 repress some promoters and activate others. Isoform OCT2.7 is unable to bind to the octamer motif, but can still activate the beta-casein gene promoter at low levels.

Gene ID: 18987

UniProt: Q00196