Recombinant BUB1B antibody

Catalog No. ABIN7797805

The Rabbit Monoclonal anti-BUB1B antibody (Clone 23GB1640) (ABIN7797805) specifically detects BUB1B in WB, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for Recombinant BUB1B antibody (ABIN7797805)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: KD Validated

Clone: 23GB1640

Product Details anti-BUB1B Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated BubR1 Recombinant Rabbit mAb

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human BubR1

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunocytochemistry (ICC): 1:100-1:1,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BubR1

Molecular Weight: 120 kDa

Gene ID: 701

UniProt: O60566