Recombinant Dihydrofolate Reductase antibody

Catalog No. ABIN7798334

This Rabbit Monoclonal antibody specifically detects Dihydrofolate Reductase in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Dihydrofolate Reductase antibody (ABIN7798334)

Target: Dihydrofolate Reductase (DHFR)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 23GB3575

Product Details anti-Dihydrofolate Reductase Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Dihydrofolate reductase Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human DHFR

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: Dihydrofolate reductase

Molecular Weight: 21 kDa

Gene ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases