Recombinant ENO1 antibody

Catalog No. ABIN7798448

This Rabbit Monoclonal antibody specifically detects ENO1 in WB, FACS and IF (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant ENO1 antibody (ABIN7798448)

Target: ENO1 (Enolase 1 (ENO1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 25GB2820

Product Details anti-ENO1 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Enolase 1 Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human ENO1

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: Enolase 1

Molecular Weight: 47 kDa

Gene ID: 2023

UniProt: P06733