Recombinant EIF2A antibody (pSer51)

Catalog No. ABIN7798512

The Rabbit Monoclonal anti-EIF2A antibody has been validated for WB, FACS and IF (p). It is suitable to detect EIF2A in samples from Human.

Quick Overview for Recombinant EIF2A antibody (pSer51) (ABIN7798512)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 24GB3905

Product Details anti-EIF2A Antibody

Binding Specificity: pSer51

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-eIF2 alpha (Ser51) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-eIF2 alpha (Ser51)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: eIF2 alpha

Molecular Weight: 36 kDa

Gene ID: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly