Recombinant GLI3 antibody

Catalog No. ABIN7798721

This Rabbit Monoclonal antibody specifically detects GLI3 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant GLI3 antibody (ABIN7798721)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 24GB6105

Product Details anti-GLI3 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Gli3 Recombinant Rabbit mAb

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Gli3

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: Gli3

Molecular Weight: 170 kDa

Gene ID: 2737

UniProt: P10071

Pathways: Hedgehog Signaling