Recombinant Histone H3.3 antibody (H3S31ph)

Catalog No. ABIN7798830

The Rabbit Monoclonal anti-Histone H3.3 antibody has been validated for WB, FACS and IF (p). It is suitable to detect Histone H3.3 in samples from Human.

Quick Overview for Recombinant Histone H3.3 antibody (H3S31ph) (ABIN7798830)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 25GB2465

Product Details anti-Histone H3.3 Antibody

Binding Specificity: H3S31ph

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-Histone H3.3 (S31) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-Histone H3.3 (S31)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Molecular Weight: 15 kDa

Gene ID: 3020

UniProt: P84243