SKP2 antibody (AA 351-424) (Biotin)

Catalog No. ABIN737603

The Rabbit Polyclonal anti-SKP2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect SKP2 in samples from Human, Mouse and Rat.

Quick Overview for SKP2 antibody (AA 351-424) (Biotin) (ABIN737603)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SKP2 Antibody (Biotin)

Binding Specificity: AA 351-424

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Skp2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2

Background:

Synonyms: p45, FBL1, FLB1, FBXL1, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2, SKP2

Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Gene ID: 6502

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases