Recombinant PRKAA1 antibody (pSer496)

Catalog No. ABIN7800008

This Rabbit Monoclonal antibody specifically detects PRKAA1 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant PRKAA1 antibody (pSer496) (ABIN7800008)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 23GB2660

Product Details anti-PRKAA1 Antibody

Binding Specificity: pSer496

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-AMPK alpha 1 (S496) Recombinant Rabbit mAb

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-AMPK alpha 1 (S496)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: AMPK alpha 1

Molecular Weight: 64 kDa

Gene ID: 5562

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect