Recombinant Sonic Hedgehog Signaling Molecule antibody

Catalog No. ABIN7800969

The Rabbit Monoclonal anti-Sonic Hedgehog Signaling Molecule antibody is suitable to detect Sonic Hedgehog Signaling Molecule in samples from Human. It has been validated for FACS, IF (p) and WB.

Quick Overview for Recombinant Sonic Hedgehog Signaling Molecule antibody (ABIN7800969)

Target: Sonic Hedgehog Signaling Molecule

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Western Blotting (WB)

Grade: KD Validated

Clone: 24GB1925

Product Details

Expression System: HEK-293 Cells

Purpose: KD-Validated Sonic Hedgehog Signaling Molecule Recombinant Rabbit mAb

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Sonic Hedgehog

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details

Target: Sonic Hedgehog Signaling Molecule

Molecular Weight: 50 kDa

Gene ID: 6469

UniProt: Q15465