OCT4 antibody (AA 201-300) (Biotin)

Catalog No. ABIN737828

The Rabbit Polyclonal anti-OCT4 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect OCT4 in samples from Human, Mouse and Rat.

Quick Overview for OCT4 antibody (AA 201-300) (Biotin) (ABIN737828)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-OCT4 Antibody (Biotin)

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse OCT-4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct-4

Background:

Synonyms: Oct3, Oct4, Otf3, Otf4, NF-A3, Oct-3, Oct-4, Otf-3, Otf-4, OCT 4, Otf3g, Oct3/4, Oct-3/4, Otf3-rs7, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Pou5f1

Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Gene ID: 18999

UniProt: P20263

Pathways: Stem Cell Maintenance