MBP antibody (AA 69-85)

Catalog No. ABIN739400

The Rabbit Polyclonal anti-MBP antibody has been validated for WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect MBP in samples from Human, Rat and Mouse. There are 6+ publications available.

Quick Overview for MBP antibody (AA 69-85) (ABIN739400)

Target: MBP

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MBP Antibody

Binding Specificity: AA 69-85

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Guinea Pig MBP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MBP antibody (ABIN739400)

Luo, Wei, Wang, Wang: "Paternal bisphenol a diet changes prefrontal cortex proteome and provokes behavioral dysfunction in male offspring." in: Chemosphere, Vol. 184, pp. 720-729, (2017) (PubMed).

Zhang, Gong, Qiu, Zhang, Gong: "MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 2809-2816, (2017) (PubMed).

Gao, Li, Zheng, Guan, Ma: "Isolation of a pluripotent neural stem cell from the embryonic bovine brain." in: International journal of molecular sciences, Vol. 16, Issue 3, pp. 5990-9, (2015) (PubMed).

Mori, Matsubara, Matsubara, Uchikura, Hashimoto, Fujioka, Matsumoto: "Stromal Cell-Derived Factor-1? Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 18018-32, (2015) (PubMed).

Liu, Chen, Liu, Yang, Xie, Zheng, Xu, Chen, Wang, Zhang, Bai, Jin: "Acellular spinal cord scaffold seeded with mesenchymal stem cells promotes long-distance axon regeneration and functional recovery in spinal cord injured rats." in: Journal of the neurological sciences, Vol. 325, Issue 1-2, pp. 127-36, (2013) (PubMed).

Target Details for MBP

Target: MBP

Background:

Synonyms: MBP_CAVPO,Myelin Basic Protein, Myelin basic protien, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MBP, MGC99675, MLD, Myelin A1 Protein, Myelin Deficient, Myelin Membrane Encephalitogenic Protein, SHI, Shiverer, SP, MBP TAG, MBP-TAG

Background: Oligodendrocyte Marker The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.