CYP7A1 antibody (AA 351-400)

Catalog No. ABIN739725

Product Details anti-CYP7A1 Antibody

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Binding Specificity: AA 351-400

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP7A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Rat

Predicted Reactivity: Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CYP7A1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CYP7A1 antibody (ABIN739725)

Zhang, Wang, Hao, Wang, Zhang, Muhammad, Qi, Li, Sun: "Nrf2 is crucial for the down-regulation of Cyp7a1 induced by arachidonic acid in Hepg2 cells." in: Environmental toxicology and pharmacology, Vol. 52, pp. 21-26, (2017) (PubMed).

Guo, Hassan, Zhang, Dong, Ding, Wang, Sun, Zhang, Jiang: "Pyrazinamide Induced Rat Cholestatic Liver Injury through Inhibition of FXR Regulatory Effect on Bile Acid Synthesis and Transport." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 152, Issue 2, pp. 417-28, (2016) (PubMed).

Kou, Han, Wang, Huang, He, Han, Zhou, Ye, Li: "Synergetic cholesterol-lowering effects of main alkaloids from Rhizoma Coptidis in HepG2 cells and hypercholesterolemia hamsters." in: Life sciences, Vol. 151, pp. 50-60, (2016) (PubMed).

Nukaya, Minami, Hoshikawa, Yokoi, Seino: "Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 20, Issue 5, pp. 367-81, (2015) (PubMed).

Wang, Xiaoling, Pingting, Shuqiang, Yuaner: "Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats." in: Lipids in health and disease, Vol. 13, pp. 77, (2014) (PubMed).

Wu, He, Wang, Xue, Ning, Zou, Ye, Li, Wang, Pang: "The antihypercholesterolemic effect of jatrorrhizine isolated from Rhizoma Coptidis." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 21, Issue 11, pp. 1373-81, (2014) (PubMed).

Gabbi, Bertolotti, Anzivino, Macchioni, Del Puppo, Ricchi, Carubbi, Tagliafico, Romagnoli, Odoardi, Loria, Losi, Carulli: "Effects of bile duct ligation and cholic acid treatment on fatty liver in two rat models of non-alcoholic fatty liver disease." in: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, Vol. 44, Issue 12, pp. 1018-26, (2012) (PubMed).

Target Details for CYP7A1

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Alternative Name: CYP7A1 (CYP7A1 Products)

Background:

Synonyms: CP7A, CYP7, CYPVII, Cholesterol 7-alpha-monooxygenase, Cholesterol 7-alpha-hydroxylase, Cytochrome P45 7A1, CYP7A1

Background: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.

Gene ID: 1581

UniProt: P22680

Pathways: Steroid Hormone Biosynthesis, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha