VAMP2 antibody (N-Term) (Atto 594)

Catalog No. ABIN7820739

This Mouse Monoclonal antibody specifically detects VAMP2 in ICC. It exhibits reactivity toward Human, Mouse, Rat and Hamster.

Quick Overview for VAMP2 antibody (N-Term) (Atto 594) (ABIN7820739)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP2 antibody is conjugated to Atto 594

Application: Immunocytochemistry (ICC)

Grade: KO Validated

Clone: 69-1

Product Details anti-VAMP2 Antibody (Atto 594)

Binding Specificity: N-Term

Purpose: Mouse anti-Synaptobrevin2 IgG

Cross-Reactivity (Details): Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster. No signal: chicken, zebrafish. Other species not tested yet.

Characteristics: fluorescence-labeled with ATTO®594. For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used. ATTO® 488: λex 500 nm / λem 520 nm ATTO® 550: λex 554 nm / λem 576 nm ATTO® 565: λex 564 nm / λem 590 nm ATTO® 594: λex 603 nm / λem 626 nm ATTO® 647N: λex 646 nm / λem 664 nm ATTO®488,ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. ATTO®488,ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

Purification: purified IgG

Immunogen: Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)

Isotype: IgG1

Application Details

Application Notes: WB:N/A IP:N/A ICC:1 : 500 up to 1 : 1000, IHC:not tested yet IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Concentration: 1 mg/mL

Buffer: PBS

Preservative: Without preservative

Handling Advice: Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. Reconstitute immediately upon receipt!

Storage: -20 °C,-80 °C

Storage Comment: Albumin was added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: Synaptobrevin2

Background:

Synonyms: VAMP 2, p18-2, pain

Antigen Information: K.O. Synaptobrevin 2 also known as Vamp 2 is a major vesicle protein involved in fusion

UniProt: P63045

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport