Feimin antibody

Catalog No. ABIN7822917

Supplier Product No.: 200-401-nh2

The Rabbit Polyclonal anti-Feimin antibody is suitable to detect Feimin in samples from Human and Mouse. It has been validated for ELISA, IP and WB.

Quick Overview for Feimin antibody (ABIN7822917)

Target: Feimin

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Supplier Product No.: 200-401-nh2

Supplier: Rockland

Purpose: Anti-Feimin (RABBIT) Antibody

Cross-Reactivity (Details): A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.

Purification: Anti-Feimin Antibody is purified from monospecific antiserum by Protein A affinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant mouse Feimin protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes: Anti-Feimin (RABBIT) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T and E. coli). A band approximately 20.1kDa in size corresponding to C5orf24 is detected. Cell lines expressing feimin can be expected using appropriate tissues and lysates. Specific conditions for reactivity should be optimized by the end user.

Comment:

ELISA Dilution: 1:44,500-1:64,500
Western Blot Dilution: 40 ng/mL
Immunoprecipitation Dilution: 40 ng/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.18 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Feimin

Background: Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin

Gene ID: 134553

NCBI Accession: NP_001129058.1

UniProt: Q80X32