KCC2 antibody (C-Term, Intracellular)

Catalog No. ABIN7822998

The Mouse Monoclonal anti-KCC2 antibody (Clone N1-66) (ABIN7822998) specifically detects KCC2 in IHC, WB, ELISA and ICC. The antibody is reactive with Rat samples.

Quick Overview for KCC2 antibody (C-Term, Intracellular) (ABIN7822998)

Target: KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: N1-66

Product Details anti-KCC2 Antibody

Binding Specificity: AA 932-1043, C-Term, Intracellular

Purpose: Anti-KCC2 Antibody (BSA and Azide free)

Specificity: No off-targets reported for KCC1, KCC3 or KCC4

Cross-Reactivity: Chicken, Human, Mouse

Characteristics: Our Anti-KCC2 mouse monoclonal primary antibody is produced in-house from hybridoma clone N1/66. It detects chicken, human, mouse, and rat KCC2, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b (accession number Q63633) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS7.4, carrier free

Preservative: Azide free

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 12 months

Target Details for KCC2

Target: KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))

Alternative Name: KCC2

Background:

Synonyms: Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter)

Description Logic: Our Anti-KCC2 mouse monoclonal primary antibody is produced from hybridoma clone N1/66. It detects chicken, human, mouse, and rat KCC2, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Gene Name Alternatives: Slc12a5 Kcc2 Kiaa1176

Molecular Weight: 140 kDa

UniProt: Q63633