Histone H3.3 antibody (AA 83-93)

Catalog No. ABIN7823378

The Mouse Monoclonal anti-Histone H3.3 antibody has been validated for ELISA and IHC. It is suitable to detect Histone H3.3 in samples from Rat.

Quick Overview for Histone H3.3 antibody (AA 83-93) (ABIN7823378)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: N158-28

Product Details anti-Histone H3.3 Antibody

Binding Specificity: AA 83-93

Purpose: Anti-Histone H3.3 Antibody (BSA and Azide free)

Sequence: LRFQSAAIGA L

Specificity: No off-targets reported for Histone H3.1

Cross-Reactivity: Human, Mouse

Characteristics: Our Anti-Histone H3.3 mouse monoclonal primary antibody is produced in-house from hybridoma clone N158/28. It detects human, mouse, and rat Histone H3.3, and is purified by protein a chromatography. It is great for use in IHC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Synthetic peptide amino acids 83-93 (LRFQSAAIGAL) of rat Histone H3.3 (accession number P84245)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS7.4, carrier free

Preservative: Azide free

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 12 months

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Background:

Synonyms: Histone H3.3

Description Logic: Our Anti-Histone H3.3 mouse monoclonal primary antibody is produced from hybridoma clone N158/28. It detects human, mouse, and rat Histone H3.3, and is purified by protein a chromatography. It is great for use in IHC, ELISA.

Gene Name Alternatives: H3f3b H3.3b

Molecular Weight: 15 kDa

UniProt: P84245