Notch1 antibody (Extracellular, N-Term)

Catalog No. ABIN7823623

This Mouse Monoclonal antibody specifically detects Notch1 in WB, ELISA and ICC. It exhibits reactivity toward Mouse.

Quick Overview for Notch1 antibody (Extracellular, N-Term) (ABIN7823623)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: N253-32

Product Details anti-Notch1 Antibody

Binding Specificity: AA 20-216, Extracellular, N-Term

Purpose: Anti-Notch1 Antibody (BSA and Azide free)

Specificity: No off-targets reported for Notch2 or Notch3

Cross-Reactivity: Rat

Characteristics: Our Anti-Notch1 mouse monoclonal primary antibody is produced in-house from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (accession number Q01705) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS7.4, carrier free

Preservative: Azide free

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 12 months

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch1

Background:

Synonyms: Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT), Notch 1 intracellular domain (NICD)]

Description Logic: Our Anti-Notch1 mouse monoclonal primary antibody is produced from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Gene Name Alternatives: Notch1 Motch

Molecular Weight: >270 kDa, ~120 kDa and smaller fragments (due to proteolysis)

UniProt: Q01705

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development