GFAP antibody (AA 411-422)

Catalog No. ABIN7824504

The Mouse Monoclonal anti-GFAP antibody (Clone N206A-8) (ABIN7824504) specifically detects GFAP in WB, IHC, ICC and ELISA. The antibody is reactive with Human samples.

Quick Overview for GFAP antibody (AA 411-422) (ABIN7824504)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Grade: KO Validated

Clone: N206A-8

Product Details anti-GFAP Antibody

Binding Specificity: AA 411-422

Purpose: Anti-GFAP Antibody

Sequence: KTVEMRDGEV IK

Specificity: Recognizes GFAP-R416W and other GFAP mutant proteinsNo off-targets reported for other proteins (based on KO validation results)

Cross-Reactivity: Drosophila melanogaster, Guinea Pig, Mouse, Polar bear (Ursus maritimus), Rat

Characteristics: Our Anti-GFAP mouse monoclonal primary antibody is produced in-house from hybridoma clone N206A/8. It is KO validated, detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Synonyms: Glial fibrillary acidic protein (GFAP)

Description Logic: Our Anti-GFAP mouse monoclonal primary antibody is produced from hybridoma clone N206A/8. It is KO validated, detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Gene Name Alternatives: GFAP

Molecular Weight: 50 kDa

UniProt: P14136