MFF antibody (Cytosolic, N-Term)

Catalog No. ABIN7824558

This anti-MFF antibody is a Mouse Monoclonal antibody detecting MFF in WB, ELISA, IHC and ICC. Suitable for Human.

Quick Overview for MFF antibody (Cytosolic, N-Term) (ABIN7824558)

Target: MFF (Mitochondrial Fission Factor (MFF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: N382-14

Product Details anti-MFF Antibody

Binding Specificity: AA 1-173, Cytosolic, N-Term

Purpose: Anti-MFF Antibody

Sequence: MSKRTSSDTP LGRVSGAAFP SPTASEMAEI SRIQYEMEYT EGIS,QRMRVPEKLK VAPPNADLEQ GFQEGVPNAS VIMQVPERIV VAGNNEDVSF SRPADLDLIQ S,TPFKPLALKT PPRVLTLSER PLDFLDLERP PVTPQNEEIR AVGRLKRERS MSENAVRQNG QL,VRNDSV,YGISNIEATI EGTSDDM,TVVDAASLRR QIIKLNRRLQ LLEEENKERA KREM

Specificity: No off-targets reported

Cross-Reactivity: Mouse, Rat

Characteristics: Our Anti-MFF mouse monoclonal primary antibody is produced in-house from hybridoma clone N382/14. It is KO validated, detects human, mouse, and rat MFF, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 1-173 (MSKRTSSDTPLGRVSGAAFPSPTASEMAEISRIQYEMEYTEGIS QRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQS TPFKPLALKTPPRVLTLSERPLDFLDLERPPVTPQNEEIRAVGRLKRERSMSENAVRQNGQL VRNDSV, cytoplasmic N-terminal exons 1, 2, 3 and 4) and 272-322 (YGISNIEATIEGTSDDM TVVDAASLRRQIIKLNRRLQLLEEENKERAKREM, cytoplasmic N-terminal exons 8 and most of 9) of mostly human MFF (also known as Mitochondrial fission factor, C2orf33, AD030, AD033 and GL004, accession number Q9GZY8), Human: 96% identity (167/173 amino acids identical) and 94% identity (48/51 amino acids identical), Rat: 95% identity (141/147 amino acids identical) and 92% identity (47/51 amino acids identical), Mouse: 93% identity (138/147 amino acids identical) and 84% identity (43/51 amino acids identical)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 24 months

Target Details for MFF

Target: MFF (Mitochondrial Fission Factor (MFF))

Alternative Name: MFF

Background:

Synonyms: Mitochondrial fission factor

Description Logic: Our Anti-MFF mouse monoclonal primary antibody is produced from hybridoma clone N382/14. It is KO validated, detects human, mouse, and rat MFF, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Gene Name Alternatives: MFF C2orf33 AD030 AD033 GL004

Molecular Weight: 40 kDa

UniProt: Q9GZY8