HLCS antibody (AA 14-654)

Catalog No. ABIN7825287

This Rabbit Polyclonal antibody specifically detects HLCS in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HLCS antibody (AA 14-654) (ABIN7825287)

Target: HLCS (Biotin-Protein Ligase (HLCS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLCS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-HLCS Antibody

Binding Specificity: AA 14-654

Purpose: Anti-HLCS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HLCS Antibody catalog # A02377-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HLCS recombinant protein (Position: Q14-K654). Human HLCS shares 75.9% amino acid (aa) sequence identity with mouse HLCS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HLCS

Target: HLCS (Biotin-Protein Ligase (HLCS))

Alternative Name: HLCS

Background: This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 81 kDa

Gene ID: 3141

UniProt: P50747