EFS antibody (AA 183-553)

Catalog No. ABIN7825352

The Rabbit Polyclonal anti-EFS antibody (ABIN7825352) specifically detects EFS in WB, ELISA, IP and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EFS antibody (AA 183-553) (ABIN7825352)

Target: EFS (Embryonal Fyn-Associated Substrate (EFS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EFS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-EFS Antibody

Binding Specificity: AA 183-553

Purpose: Anti-EFS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EFS Antibody catalog # A00572. Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EFS recombinant protein (Position: E183-T553). Human EFS shares 82.2% amino acid (aa) sequence identity with mouse EFS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for EFS

Target: EFS (Embryonal Fyn-Associated Substrate (EFS))

Alternative Name: EFS

Background: The protein encoded by this gene is a member of the CAS (CRK-associated substrate) family of adaptor proteins which typically serve as scaffolds for the assembly of larger signaling complexes. These complexes form at the cell surface where integrin binding leads to the subsequent phosphorylation of a CAS protein. Additional binding of SRC family kinases leads to CAS hyperphosphorylation and the creation of binding sites for CRK and other proteins that cause actin cytoskeleton reorganization. This gene plays a role in integrin-mediated cell attachment, spreading, and migration and also plays a role in both normal and malignant cellular transformation. This broadly expressed gene has been shown to play a role in neurite outgrowth and its expression in the thymus and lymphocytes is important for T cell maturation and the development of immunological self-tolerance. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 85 kDa

Gene ID: 10278

UniProt: O43281