EIF4A2 antibody (AA 1-119)

Catalog No. ABIN7825366

The Rabbit Polyclonal anti-EIF4A2 antibody has been validated for WB, IHC and IF. It is suitable to detect EIF4A2 in samples from Zebrafish (Danio rerio).

Quick Overview for EIF4A2 antibody (AA 1-119) (ABIN7825366)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF4A2 Antibody

Binding Specificity: AA 1-119

Purpose: Anti-Zebrafish EIF4A2 Antibody

Characteristics: Anti-Zebrafish-EIF4A2-Antibody catalog # AZF1R166. Tested in WB, IHC, IF applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish EIF4A2 recombinant protein (Position: M1-Q119).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish
Immunohistochemistry, 2-5 μg/mL, Zebrafish
Immunofluorescence, 5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2

Background: Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.

UniProt: F1R166