EIF4A3 antibody (AA 14-411)
Quick Overview for EIF4A3 antibody (AA 14-411) (ABIN7825367)
Target
See all EIF4A3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 14-411
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Purpose
- Anti-EIF4A3 Antibody
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Cross-Reactivity (Details)
- No cross-reactivity with other proteins
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Characteristics
- Anti-EIF4A3 Antibody catalog # A03095-2. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human EIF4A3 recombinant protein (Position: R14-I411). Human EIF4A3 shares 99.7% and 100% amino acid (aa) sequence identity with mouse and rat EIF4A3, respectively.
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Isotype
- IgG
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Application Notes
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))
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Alternative Name
- EIF4A3
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Background
- This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
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Molecular Weight
- 47 kDa
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Gene ID
- 9775
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UniProt
- P38919
Target
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